Publications

Alexander Apostle, Manoj Perera, Daniel Middleton, Shiyue Fang. Sensitive RNA Synthesis Using Fluoride-Cleavable Groups for Linking and Amino Protection. ChemRxiv [preprint] 2024, open access

Yipeng Yin, Reed Arneson, Yinan Yuan, Shiyue Fang. Long Oligos: Direct Chemical Synthesis of Genes with up to 1,728 Nucleotides. ChemRxiv [preprint] 2024, open access

Shiyue Fang, Reed Arneson, Yipeng Yin, Yinan Yuan. De Novo Synthesis of Error-free Long Oligos. Current Protocols 2024, doi:10.1002/cpz1.70028

Yipeng Yin, Komal Chillar, Alexander Apostle, Bhaskar Halami, Adikari M. D. N. Eriyagama, Marina Tanasova, Shiyue Fang.  For Catching-by-Polymerization Oligo Purification: Scalable Synthesis of the Precursors to the Polymerizable Tagging Phosphoramidites. PeerJ Organic Chemistry 2024, 6, e12. open access. ChemRxiv [preprint], open access

Alexander Apostle, Shiyue Fang. Deprotection of m1A Containing RNA Using Triethylamine Hydrogen Fluoride. Nucleosides, Nucleotides, and Nucleic Acids 2024, 1-8

(Invited publication) Shiyue Fang, Komal Chillar, Yipeng Yin, Alexander Apostle, Dhananjani Eriyagama, Shahien Shahsavari, Bhaskar Halami, Yinan Yuan. Oligodeoxynucleotide Synthesis under Non-Nucleophilic Deprotection Conditions. Current Protocols 2024, 4, doi:10.1002/cpz1.983. NSF-PAR

Alexander Apostle, Reed Arneson, Yinan Yuan, Shiyue Fang. The Naturally Occurring m1A RNA Modification Can Be Efficiently Incorporated into RNA by SARS-CoV-2 RdRp. Research Square [preprint] 2023, open access; or Preprints, open access

Yipeng Yin,+ Reed Arneson,+ Alexander Apostle, Adikari Eriyagama, Komal Chillar, Emma Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang. Long Oligodeoxynucleotides: Chemical Synthesis, Isolation via Catching by Polymerization, Verification via Sequencing, and Gene Expression Demonstration. Beilstein Journal of Organic Chemistry 2023, 19, 1957-1965; open access. ChemRxiv [preprint], open access (+ equal contributors)

Komal Chillar, Yipeng  Yin, Alexander Apostle, Shiyue Fang. PEGylated Dmoc Phosphoramidites for Sensitive Oligodeoxynucleotide Synthesis. Organic and Biomolecular Chemistry 2023, 21, 9005-9010. PubMed

Komal Chillar,+ Dhananjani N. A. M. Eriyagama,+ Yipeng  Yin,+ Shahien Shahsavari,+ Bhaskar Halami, Alexander Apostle, Shiyue Fang. Oligonucleotide Synthesis under Mild Deprotection Conditions. New Journal of Chemistry 2023478714 – 8722. ChemRxiv [preprint], open access (equal contributors)

Alexander Apostle, Yipeng Yin, Komal Chillar, Adikari M. D. N. Eriyagama, Reed Arneson, Emma Burke, Shiyue Fang, Yinan Yuan. Effects of Epitranscriptomic RNA Modifications on the Catalytic Activity of SARS-CoV-2 Replication Complex. ChemBioChem 202324e2023000ChemRxiv [preprint], open access

Alexander Apostle, Shiyue Fang. Dissolve-Spin: Desalting Oligonucleotides for MALDI MS Analysis. Journal of Mass Spectrometry 202257e4893. NSF-PAR. PubMed

Komal Chillar, Yipeng Yin, Dhananjani N. A. M. Eriyagama, Shiyue Fang. Determination of Optical Density (OD) of Oligodeoxynucleotides from HPLC Peak Area. PeerJ Analytical Chemistry 20224, e20; open access. ChemRxiv [preprint], open access

Dhananjani N. A. M. Eriyagama, Yipeng Yin, Shiyue Fang. Automated Stepwise PEG Synthesis Using a Base-Labile Protecting Group. Tetrahedron 2022119132861. NSF-PAR

Logan Mikesell, Dhananjani N. A. M. Eriyagama,+ Yipeng Yin,+ Bao-Yuan Lu, Shiyue Fang. Stepwise PEG Synthesis Featuring Deprotection and Coupling in One Pot. Beilstein Journal of Organic Chemistry 202117, 2976-2982; open access (equal contributors)

Shiyue Fang. The Ion Pair Thermal Model of MALDI MS. ChemRxiv [preprint] 2021, October, 19; open access

(Invited publication) Shiyue Fang, Dhananjani Eriyagama, Yinan Yuan, Shahien Shahsavari, Jinsen Chen, Xi Lin, Bhaskar Halami. Dim and Dmoc Protecting Groups for Oligodeoxynucleotide Synthesis. Current Protocols in Nucleic Acid Chemistry 202082e111. NSF-PAR

Bhaskar Halami, Dhananjani N. A. M. Eriyagama, Komal Chillar, Zack Nelson, Lucas Prehoda, Yipeng Yin, Bao-Yuan Lu, Brett Otto, Liam Haggerty, Shiyue Fang. Linear Oligosulfoxides: Synthesis and Solubility Studies. Tetrahedron Letters 201960151306. PubMed

Shahien Shahsavari, Dhananjani Eriyagama, Jinsen Chen, Bhaskar Halami, Yipeng Yin, Komal Chillar, Shiyue Fang. Sensitive Oligodeoxynucleotide Synthesis Using Dim and Dmoc as Protecting Groups. The Journal of Organic Chemistry 20198413374-13383. open access PMC6825528

Shahien Shahsavari, Dhananjani N. A. M. Eriyagama, Bhaskar Halami, Vagarshak Begoyan, Marina Tanasova, Jinsen Chen, Shiyue Fang. Electrophilic Oligodeoxynucleotide Synthesis using dM-Dmoc for Amino Protection. Beilstein Journal of Organic Chemistry 201915, 1116-1128; open access

Dhananjani N. A. M. Eriyagama, Shahien Shahsavari, Bhaskar Halami, Bao-Yuan Lu, Fengping Wei, Shiyue Fang. Parallel, Large Scale and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-length Sequence by Polymerization Technique. Organic Process Research and Development 2018221282-1288. NSF-PAR

Bhaskar Halami, Shahien Shahsavari, Zack Nelson, Lucas Prehoda, Dhananjani N. A. M. Eriyagama, Shiyue Fang. Incorporation of Sensitive Ester and Chloropurine Groups into Oligodeoxynucleotides through Solid Phase Synthesis. ChemistrySelect 201838857-8862. PubMed

Shahien Shahsavari, Chase McNamara, Mark Sylvester, Emily Bromley, Savannah Joslin, Bao-Yuan Lu, Shiyue Fang. Amine Protecting Groups Deprotectable under Nearly Neutral Oxidative Conditions. Beilstein Journal of Organic Chemistry 201814, 1750-1757; open access

Shahien Shahsavari, Travis Wigstrom, James Gooding, Chase McNamara, Shiyue Fang. dM-Dim for Carboxylic Acid Protection. Tetrahedron Letters 2018591763-1766. PubMed

Ashok Khanal, Shiyue Fang. Solid Phase Stepwise Synthesis of Polyethylene Glycol. Chemistry—A European Journal 2017231533-1542. PubMed

Shahien Shahsavari, James Gooding, Travis Wigstrom, Shiyue Fang. Formation of Hindered Arylcarbamates using Alkyl Aryl Carbonates under Highly Reactive Conditions. ChemistrySelect 201723959-3963. PubMed

Xi Lin,+ Jinsen Chen,+ Shahien Shahsavari,+ Nathanael Green, Deepti Goyal, Shiyue Fang. Synthesis of Oligodeoxynucleotides Containing Electrophilic Groups. Organic Letters 2016183870-3873 (+ equal contributors). PubMed

Shahien Shahsavari,+ Jinsen Chen,+ Travis Wigstrom, James Gooding, Alexander Gauronskas, Shiyue Fang. Tritylation of Alcohols under Mild Conditions without Using Silver Salts. Tetrahedron Letters 2016573877-3880 (equal contributors)

Ashok Khanal,+ Fei Long,+ Bin Cao, Reza Shahbazian-Yassar, Shiyue Fang. Evidence of Splitting 1,2,3-Triazole into an Alkyne and Azide by Low Mechanical Force in the Presence of Other Covalent Bonds. Chemistry—A European Journal 2016229760-9767. (+ equal contributors). open access

Durga Pokharel, Shiyue Fang. Polymerizable Phosphoramidites with Acid-Cleavable Linker for Eco-Friendly Synthetic Oligodeoxynucleotide Purification. Green Chemistry 2016181125-1136. open access. open access

Fei Long, Bin Cao, Ashok Khanal, Shiyue Fang, Reza Shahbazian-Yassar. Modification of a Single-Molecule AFM Probe with Highly Defined Surface Functionality. Beilstein Journal of Nanotechno​logy 20145, 2122-2128; open access

Durga Pokharel, Shiyue Fang. A Highly Convenient Procedure for Oligodeoxynucleotide Purification. The Open Organic Chemistry Journal 20148, 15-18; open access

Durga Pokharel, Suntara Fueangfung, Mingcui Zhang, Shiyue Fang. Peptide and Peptide Nucleic Acid Syntheses Using a DNA/RNA Synthesizer. Biopolymers: Peptide Science 2014102487-493

Mingcui Zhang, Durga Pokharel, Shiyue Fang. Purification of Synthetic Peptides Using a Catching Full-length Sequence by Polymerization Approach. Organic Letters 2014161290-1293

Suntara Fueangfung, Yinan Yuan, Shiyue Fang. Denaturing Reversed-Phase HPLC Using a Mobile Phase Containing Urea for Oligodeoxynucleotide Analysis. Nucleosides, Nucleotides and Nucleic Acids 201433481-488

Durga Pokharel, Yinan Yuan, Suntara Fueangfung, Shiyue Fang. Synthetic Oligodeoxynucleotide Purification by Capping Failure Sequences with a Methacrylamide Phosphoramidite Followed by Polymerization. RSC Advances 201448746–8757