Using a polymerizable phosphoramidite as the coupling agent in the last synthetic cycle of automated oligodeoxynucleotide (ODN) synthesis, a polymerizable group (i.e. methacrylamide group) can be attached to the full-length ODN (but not to the failure ODNs). After cleavage and deprotection, the full-length ODN is co-polymerized into an insoluble polyacrylamide gel (the failure ODNs are not). The failure ODNs and other impurities are washed away with water. The full-length ODN is then cleaved and extracted from the gel.
- Capable of isolation of full-length ODN from complex crude mixture
- Useful for the synthesis and purification of long ODNs
- Synthesis and purification of up to 400-mer has been demonstrated
- Overcoming challenges in the synthesis of genes containing long repeats and stable higher order structures that cannot be overcome using traditional PCR assembly methods
Molecular Formula: C59H80N9O12P
Molecular Weight: 1138.31
CAS Number: 1818326-10-1
Purity: >95% (1H NMR)
Solubility: soluble in CH3CN and CH2Cl2
Recommended Storage Temperature: –20 oC or lower
Shipping Temperature: room temperature
Suggested Amount per Experiment: 25 µmol (29 mg) for each 1 µmol oligo synthesis
References:
- ChemRxiv [preprint] 2023 doi:10.26434/chemrxiv-2023-79qj3
- Org Process Res Dev 2018 22 1282-1288 doi:10.1021/acs.oprd.8b00209
- Green Chem 2016 18 1125-1136 doi:10.1039/c5gc01762a
- Org Lett 2014 16 1290-1293 doi:10.1021/ol403426u