{"id":33,"date":"2024-02-24T21:30:15","date_gmt":"2024-02-24T21:30:15","guid":{"rendered":"http:\/\/localhost:8888\/tiwarilab\/?page_id=33"},"modified":"2024-03-15T16:44:38","modified_gmt":"2024-03-15T16:44:38","slug":"funding","status":"publish","type":"page","link":"https:\/\/chem.sites.mtu.edu\/tiwari\/funding\/","title":{"rendered":"Funding"},"content":{"rendered":"\t\t
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See our External \/ Internal<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Funding<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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2022-2025\u00a0 \u00a0 \u00a0<\/td>\nAgency:<\/strong> National Institutes of Health (Grant #1 R15 GM148964-01)
Topic:<\/strong> Are all Protein Aggregates Toxic?
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> The goal of this project is to identify unique physiochemical properties of aggregates, shared by a large number of structurally diverse proteins (e.g. A\u03b242 peptide, insulin, and lysozyme), that are toxic<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n

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2019-2022<\/td>\nAgency:<\/strong> National Institutes of Health (Grant #2 R15 GM114751-02)
Topic:<\/strong> Ratiometric Near-Infrared Fluorescent Probes for Sensitive Detection of Lysosomal and Mitochondrial pH Changes in Live Cells
Principal Investigator:<\/strong> Haiying Liu, Ph.D.
Co-Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> Develop ratiometric near-infrared fluorescent probes for analysis of lysosomal and mitochondrial pH changes in live cells.<\/td>\n<\/tr>\n
2016-Present<\/td>\nAgency:<\/strong> Alumni Donation
Topic:<\/strong> The Protein Misfolding Diseases Research Fund
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> Laboratory research funds for Tiwari lab.<\/td>\n<\/tr>\n
2015-Present<\/td>\nAgency:<\/strong> Alumni Donation
Topic:<\/strong> Linda J. Horton Laboratory Research Fund
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> Laboratory research funds for Tiwari lab.<\/td>\n<\/tr>\n
2015-2019<\/td>\nAgency:<\/strong> National Institutes of Health (Grant #2 R15 GM114751-01)
Topic:<\/strong> Ratiometric Near-Infrared Fluorescent Probes for Lysosomal pH in Living Cells
Principal Investigator:<\/strong> Haiying Liu, Ph.D.
Co-Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To synthesize and test near-infrared fluorescent probes for lysosomal pH in living cells.<\/td>\n<\/tr>\n
2014-2015<\/td>\nAgency:<\/strong> MTU Research Excellence Fund
Topic:<\/strong> Understanding the Role of Protein Aggregation in Cellular Toxicity
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To analyze a structurally diverse set of aggregation-prone proteins for their shared biochemical and biophysical properties including stability, post-translational modifications, and aggregation propensity\u00a0in vitro<\/em>, and to identify their interacting partners\u00a0in vivo<\/em>.<\/td>\n<\/tr>\n
2012-2013<\/td>\nAgency:<\/strong> MTU Research Excellence Fund
Topic:<\/strong> BODIPY-based Polymeric Dyes Bearing Carbohydrate Residues for Detection of Bacteria
Principal Investigator:<\/strong> Haiying Liu, Ph.D.
Co-Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To develop a broad new class of BODIPY polymer-based ratiometric biosensors for quick, selective and sensitive detection of\u00a0E. coli\u00a0<\/em>bacteria by using fluorescence resonance energy transfer (FRET).<\/td>\n<\/tr>\n
2011-2013<\/td>\nAgency: <\/strong>ALS Therapy Alliance
Topic:<\/strong> Characterizing the Surface Hydrophobicity of ALS Mutants of SOD1 by Novel Fluorescent Probes
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To characterize abnormally expossed hydrophobic residues of SOD1 using novel fluorescent probes.<\/td>\n<\/tr>\n
2008-2009<\/td>\nAgency:<\/strong> The Angel Fund
Topic:<\/strong> Altered Metal Binding and Hydrophobic Exposure of ALS mutants of Cu\/Zn Superoxide dismutase may be key to Cellular Toxicity
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To measure the metal (copper and zinc) affinity of purified wild type and ALS mutants of Cu\/Zn superoxide dismutase (SOD1) and also define potentially toxic conformations of mutant SOD1 by mapping the exposed hydrophobic amino acids.<\/td>\n<\/tr>\n
2007-2008<\/td>\nAgency:<\/strong> The ALS Therapy Alliance
Topic:<\/strong> Mapping the Aberrant Hydrophobicity of Misfolded SOD1 Mutants that Cause ALS
Principal Investigator:<\/strong> Lawrence J. Hayward, M.D., Ph.D. UMMS
Co-Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To map specific residues within SOD1 mutants responsible for abnormal hydrophobic exposure and binding in cellular and rodent models of ALS.<\/td>\n<\/tr>\n
2005-2007<\/td>\nAgency:<\/strong> Amyotrophic Lateral Sclerosis Association (ALSA) and the ALS Therapy Alliance
Topic:<\/strong> Aberrant Hydrophobicity of ALS Mutant Cu\/Zn Superoxide Dismutase (SOD1) Variants and its Relation to Toxicity
Principal Investigator:<\/strong> Ashutosh Tiwari, Ph.D.
Objective:<\/strong> To map regions within SOD1 mutants responsible for abnormal hydrophobic binding and to investigate the nature of these interactions in cellular and rodent models of ALS.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n

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See our External \/ Internal Funding 2022-2025\u00a0 \u00a0 \u00a0 Agency: National Institutes of Health (Grant #1 R15 GM148964-01)Topic: Are all Protein Aggregates Toxic?Principal Investigator: Ashutosh Tiwari, Ph.D.Objective: The goal of this project is to identify unique physiochemical properties of aggregates, shared by a large number of structurally diverse proteins (e.g. A\u03b242 peptide, insulin, and lysozyme), […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-33","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/pages\/33","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":19,"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":493,"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/pages\/33\/revisions\/493"}],"wp:attachment":[{"href":"https:\/\/chem.sites.mtu.edu\/tiwari\/wp-json\/wp\/v2\/media?parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}