{"id":9,"date":"2017-03-02T09:19:11","date_gmt":"2017-03-02T14:19:11","guid":{"rendered":"http:\/\/chem.sites.mtu.edu\/fang\/?page_id=9"},"modified":"2026-02-22T15:42:42","modified_gmt":"2026-02-22T20:42:42","slug":"publications","status":"publish","type":"page","link":"https:\/\/chem.sites.mtu.edu\/fang\/publications\/","title":{"rendered":"Publications"},"content":{"rendered":"

Miguel A. Garcia-Campos, Joe Georgeson, Ronit Nir, Robert Reichelt, Kristin A. Fluke, Donna Matzov, Vinithra Iyer, Brett W. Burkhart, Lauren Lui, Anatoly Kustanovich, Felix Gr\u00fcnberger, Supuni Thalalla-Gamage, Shereen A. Howpay-Manage, Milan Gerovac, Nicolas Alexandre, Yuko Nobe, Jakub S. Nowak, Manoj Perera, Alexander Apostle, Shiyue Fang, Sebastian Glatt, Ghil Jona, S\u00e9bastien Ferreira-Cerca, J\u00f6rg Vogel, Masato Taoka, Jordan L. Meier, Eric Westhof, Thomas J. Santangelo, Dina Grohmann, Moran Shalev-Benami, Schraga Schwartz. Pan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome. Cell<\/em> 2025<\/strong>, doi:10.1016\/j.cell.2025.09.014<\/a><\/p>\n

Alexander Apostle, Manoj Perera, Daniel Middleton, William Wittstock, Rohith Awasthy, Yinan Yuan, Shiyue Fang. Synthesis of Sensitive RNAs Using Fluoride-Cleavable Groups as Linkers and Amino-Group Protection. Angewandte Chemie International Edition<\/em> 2025<\/strong>, e202424560<\/a>; also in hot topic, RNA<\/a>; open access<\/a>. ChemRxiv<\/em> [preprint], open access<\/a><\/p>\n

Komal Chillar, Rohith Awasthy, Marina Tanasova, Shiyue Fang. Synthesis of Sensitive Oligodeoxynucleotides Containing Acylated Cytosine, Adenine, and Guanine Nucleobases. DNA<\/em> 2025<\/strong>, 5<\/em>, 25. open access<\/a>. ChemRxiv<\/em> [preprint], open access<\/a><\/p>\n

Yipeng Yin,+<\/sup> Reed Arneson,+<\/sup> Yinan Yuan, Shiyue Fang. Long Oligos: Direct Chemical Synthesis of Genes with up to 1,728 Nucleotides. Chemical Science<\/em> 2025<\/strong>, 16<\/em>, 1966-1973; open access<\/a>; hot article collection<\/a>; 2025 most popular Chemical Science<\/em> article collection<\/a>;\u00a0ChemRxiv<\/em>, open access<\/a> (+<\/sup>equal contributors). The paper is featured in Tides Global<\/a> <\/em>and Chemistry World<\/a><\/em><\/p>\n

Shiyue Fang, Reed Arneson,+<\/sup> Yipeng Yin,+<\/sup> Yinan Yuan. De Novo Synthesis of Error-free Long Oligos. Current Protocols<\/em> 2024<\/strong>, doi:10.1002\/cpz1.70028<\/a>\u00a0(+<\/sup>equal contributors)<\/p>\n

Yipeng Yin, Komal Chillar, Alexander Apostle, Bhaskar Halami, Adikari M. D. N. Eriyagama, Marina Tanasova, Shiyue Fang.\u00a0 For Catching-by-Polymerization Oligo Purification: Scalable Synthesis of the Precursors to the Polymerizable Tagging Phosphoramidites. PeerJ Organic Chemistry<\/em> 2024<\/strong>, 6<\/em>, e12. open access<\/a>. ChemRxiv<\/em> [preprint], open access<\/a><\/p>\n

Alexander Apostle, Shiyue Fang. Deprotection of m1A Containing RNA Using Triethylamine Hydrogen Fluoride. Nucleosides, Nucleotides, and Nucleic Acids<\/em> 2024<\/strong>, 1-8<\/a><\/p>\n

(Invited publication)\u00a0Shiyue Fang, Komal Chillar, Yipeng Yin, Alexander Apostle, Dhananjani Eriyagama, Shahien Shahsavari, Bhaskar Halami, Yinan Yuan. Oligodeoxynucleotide Synthesis under Non-Nucleophilic Deprotection Conditions. Current Protocols<\/em> 2024<\/strong>, 4<\/em>, doi:10.1002\/cpz1.983<\/a>. NSF-PAR<\/a><\/p>\n

Alexander Apostle, Reed Arneson, Yinan Yuan, Shiyue Fang. The Naturally Occurring m1<\/sup>A RNA Modification Can Be Efficiently Incorporated into RNA by SARS-CoV-2 RdRp. Research Square<\/em> [preprint] 2023<\/strong>, open access<\/a>; or Preprints<\/em>, open access<\/a><\/p>\n

Yipeng Yin,+<\/sup>\u00a0Reed Arneson,+<\/sup> Alexander Apostle, Adikari Eriyagama, Komal Chillar, Emma Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang. Long Oligodeoxynucleotides: Chemical Synthesis, Isolation via Catching by Polymerization, Verification via Sequencing, and Gene Expression Demonstration. Beilstein Journal of Organic Chemistry<\/em> 2023<\/strong>, 19<\/em>, 1957-1965; open access<\/a>. ChemRxiv <\/em>[preprint],\u00a0open access<\/a>\u00a0(+<\/sup>\u00a0equal contributors)<\/p>\n

Komal Chillar, Yipeng\u00a0 Yin, Alexander Apostle,\u00a0Shiyue Fang. PEGylated Dmoc Phosphoramidites for Sensitive Oligodeoxynucleotide Synthesis. Organic and Biomolecular Chemistry<\/em> 2023<\/strong>, 21<\/em>, 9005-9010<\/a>. PubMed<\/a><\/p>\n

Komal Chillar,+<\/sup>\u00a0Dhananjani N. A. M. Eriyagama,+<\/sup>\u00a0Yipeng\u00a0 Yin,+<\/sup>\u00a0Shahien Shahsavari,+<\/sup>\u00a0Bhaskar Halami, Alexander Apostle, Shiyue Fang. Oligonucleotide Synthesis under Mild Deprotection Conditions.\u00a0New Journal of Chemistry<\/em>\u00a02023<\/strong>,\u00a047<\/em>,\u00a08714 \u2013 8722<\/a>. ChemRxiv<\/em>\u00a0[preprint],\u00a0open access<\/a>\u00a0(+\u00a0<\/sup>equal contributors)<\/p>\n

Alexander Apostle, Yipeng Yin, Komal Chillar, Adikari M. D. N. Eriyagama, Reed Arneson, Emma Burke, Shiyue Fang, Yinan Yuan. Effects of Epitranscriptomic RNA Modifications on the Catalytic Activity of SARS-CoV-2 Replication Complex. ChemBioChem<\/em>\u00a02023<\/strong>,\u00a024<\/em>,\u00a0e2023000<\/a>.\u00a0ChemRxiv<\/em>\u00a0[preprint],\u00a0open access<\/a>. The paper is featured in Chemistry Europe ChemistryViews<\/a><\/em><\/p>\n

Alexander Apostle, Shiyue Fang. Dissolve-Spin: Desalting Oligonucleotides for MALDI MS Analysis.\u00a0Journal of Mass Spectrometry<\/em>\u00a02022<\/strong>,\u00a057<\/em>,\u00a0e4893<\/a>. NSF-PAR<\/a>. PubMed<\/a><\/p>\n

Komal Chillar, Yipeng Yin, Dhananjani N. A. M. Eriyagama, Shiyue Fang. Determination of Optical Density (OD) of Oligodeoxynucleotides from HPLC Peak Area.\u00a0PeerJ Analytical Chemistry<\/em>\u00a02022<\/strong>,\u00a04<\/em>, e20; open access<\/a>. ChemRxiv<\/em>\u00a0[preprint],\u00a0open access<\/a><\/p>\n

Dhananjani N. A. M. Eriyagama, Yipeng Yin, Shiyue Fang. Automated Stepwise PEG Synthesis Using a Base-Labile Protecting Group.\u00a0Tetrahedron<\/em>\u00a02022<\/strong>,\u00a0119<\/em>,\u00a0132861<\/a>. NSF-PAR<\/a><\/p>\n

Logan Mikesell, Dhananjani N. A. M. Eriyagama,+<\/sup>\u00a0Yipeng Yin,+<\/sup>\u00a0Bao-Yuan Lu, Shiyue Fang. Stepwise PEG Synthesis Featuring Deprotection and Coupling in One Pot.\u00a0Beilstein Journal of Organic Chemistry<\/em>\u00a02021<\/strong>,\u00a017<\/em>, 2976-2982; open access<\/a>\u00a0(+\u00a0<\/sup>equal contributors)<\/p>\n

Shiyue Fang. The Ion Pair Thermal Model of MALDI MS.\u00a0ChemRxiv<\/em> [preprint]\u00a02021<\/strong>, October, 19; open access<\/a><\/p>\n

(Invited publication)\u00a0Shiyue Fang, Dhananjani Eriyagama, Yinan Yuan, Shahien Shahsavari, Jinsen Chen, Xi Lin, Bhaskar Halami. Dim and Dmoc Protecting Groups for Oligodeoxynucleotide Synthesis.\u00a0C<\/em>urrent Protocols in Nucleic Acid Chemistry<\/em>\u00a02020<\/strong>,\u00a082<\/em>,\u00a0e111<\/a>. NSF-PAR<\/a><\/p>\n

Bhaskar Halami, Dhananjani N. A. M. Eriyagama, Komal Chillar, Zack Nelson, Lucas Prehoda, Yipeng Yin, Bao-Yuan Lu, Brett Otto, Liam Haggerty, Shiyue Fang. Linear Oligosulfoxides: Synthesis and Solubility Studies.\u00a0Tetrahedron Letters<\/em>\u00a02019<\/strong>,\u00a060<\/em>,\u00a0151306<\/a>. PubMed<\/a><\/p>\n

Shahien Shahsavari, Dhananjani Eriyagama, Jinsen Chen, Bhaskar Halami, Yipeng Yin, Komal Chillar, Shiyue Fang. Sensitive Oligodeoxynucleotide Synthesis Using Dim and Dmoc as Protecting Groups.\u00a0The Journal of Organic Chemistry<\/em>\u00a02019<\/strong>,\u00a084<\/em>,\u00a013374-13383<\/a>. open access PMC6825528<\/a><\/p>\n

Shahien Shahsavari, Dhananjani N. A. M. Eriyagama, Bhaskar Halami, Vagarshak Begoyan, Marina Tanasova, Jinsen Chen, Shiyue Fang. Electrophilic Oligodeoxynucleotide Synthesis using dM-Dmoc for Amino Protection.\u00a0Beilstein Journal of Organic Chemistry<\/em>\u00a02019<\/strong>,\u00a015<\/em>, 1116-1128; open access<\/a><\/p>\n

Dhananjani N. A. M. Eriyagama, Shahien Shahsavari, Bhaskar Halami, Bao-Yuan Lu, Fengping Wei, Shiyue Fang. Parallel, Large Scale and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-length Sequence by Polymerization Technique.\u00a0Organic Process Research and Development<\/em>\u00a02018<\/strong>,\u00a022<\/em>,\u00a01282-1288<\/a>. NSF-PAR<\/a><\/p>\n

Bhaskar Halami, Shahien Shahsavari, Zack Nelson, Lucas Prehoda, Dhananjani N. A. M. Eriyagama, Shiyue Fang.\u00a0Incorporation of Sensitive Ester and Chloropurine Groups into Oligodeoxynucleotides through Solid Phase Synthesis.\u00a0ChemistrySelect<\/em>\u00a02018<\/strong>,\u00a03<\/em>,\u00a08857-8862<\/a>. PubMed<\/a><\/p>\n

Shahien Shahsavari, Chase McNamara, Mark Sylvester, Emily Bromley, Savannah Joslin, Bao-Yuan Lu,\u00a0Shiyue Fang.\u00a0Amine Protecting Groups Deprotectable under Nearly Neutral Oxidative Conditions.\u00a0Beilstein Journal of Organic Chemistry<\/em>\u00a02018<\/strong>,\u00a014<\/em>, 1750-1757; open access<\/a><\/p>\n

Shahien Shahsavari, Travis Wigstrom, James Gooding, Chase McNamara,\u00a0Shiyue Fang.\u00a0dM-Dim for Carboxylic Acid Protection.\u00a0Tetrahedron Letters<\/em>\u00a02018<\/strong>,\u00a059<\/em>,\u00a01763-1766<\/a>. PubMed<\/a><\/p>\n

Ashok Khanal,\u00a0Shiyue Fang. Solid Phase Stepwise Synthesis of Polyethylene Glycol.\u00a0Chemistry<\/em>\u2014A European Journal\u00a0<\/em>2017<\/strong>,\u00a023<\/em>,\u00a01533-1542<\/a>. PubMed<\/a><\/p>\n

Shahien Shahsavari, James Gooding, Travis Wigstrom,\u00a0Shiyue Fang. Formation of Hindered Arylcarbamates using Alkyl Aryl Carbonates under Highly Reactive Conditions.\u00a0ChemistrySelect<\/em>\u00a02017<\/strong>,\u00a02<\/em>,\u00a03959-3963<\/a>. PubMed<\/a><\/p>\n

Xi Lin,+<\/sup>\u00a0Jinsen Chen,+<\/sup>\u00a0Shahien Shahsavari,+<\/sup>\u00a0Nathanael Green, Deepti Goyal,\u00a0Shiyue Fang. Synthesis of Oligodeoxynucleotides Containing Electrophilic Groups.\u00a0Organic Letters\u00a0<\/em>2016<\/strong>,\u00a018<\/em>,\u00a03870-3873<\/a>\u00a0(+<\/sup> equal contributors). PubMed<\/a>. The paper is featured in Michigan Tech News<\/a><\/p>\n

Shahien Shahsavari,+<\/sup>\u00a0Jinsen Chen,+<\/sup>\u00a0Travis Wigstrom, James Gooding, Alexander Gauronskas,\u00a0Shiyue Fang. Tritylation of Alcohols under Mild Conditions without Using Silver Salts.\u00a0Tetrahedron Letters\u00a0<\/em>2016<\/strong>,\u00a057<\/em>,\u00a03877-3880<\/a>\u00a0(+\u00a0<\/sup>equal contributors)<\/p>\n

Ashok Khanal,+<\/sup>\u00a0Fei Long,+<\/sup>\u00a0Bin Cao, Reza Shahbazian-Yassar,\u00a0Shiyue Fang. Evidence of Splitting 1,2,3-Triazole into an Alkyne and Azide by Low Mechanical Force in the Presence of Other Covalent Bonds.\u00a0Chemistry\u2014A European Journal\u00a0<\/em>2016<\/strong>,\u00a022<\/em>,\u00a09760-9767<\/a>. (+<\/sup> equal contributors). open access<\/a><\/p>\n

Durga Pokharel,\u00a0Shiyue Fang. Polymerizable Phosphoramidites with Acid-Cleavable Linker for Eco-Friendly Synthetic Oligodeoxynucleotide Purification.\u00a0Green Chemistry\u00a0<\/em>2016<\/strong>,\u00a018<\/em>,\u00a01125-1136<\/a>. open access<\/a>. open access<\/a><\/p>\n

Fei Long, Bin Cao, Ashok Khanal, Shiyue Fang, Reza Shahbazian-Yassar. Modification of a Single-Molecule AFM Probe with Highly Defined Surface Functionality.\u00a0Beilstein Journal of Nanotechno\u200blogy\u00a0<\/em>2014<\/strong>,\u00a05<\/em>, 2122-2128; open access<\/a><\/p>\n

Durga Pokharel, Shiyue Fang. A Highly Convenient Procedure for Oligodeoxynucleotide Purification.\u00a0The Open Organic Chemistry Journal\u00a0<\/em>2014<\/strong>,\u00a08<\/em>, 15-18; open access<\/a><\/p>\n

Durga Pokharel, Suntara Fueangfung, Mingcui Zhang, Shiyue Fang. Peptide and Peptide Nucleic Acid Syntheses Using a DNA\/RNA Synthesizer.\u00a0Biopolymers: Peptide Science\u00a0<\/em>2014<\/strong>,\u00a0102<\/em>,\u00a0487-493<\/a><\/p>\n

Mingcui Zhang, Durga Pokharel, Shiyue Fang. Purification of Synthetic Peptides Using a Catching Full-length Sequence by Polymerization Approach.\u00a0Organic Letters\u00a0<\/em>2014<\/strong>,\u00a016<\/em>,\u00a01290-1293<\/a><\/p>\n

Suntara Fueangfung, Yinan Yuan, Shiyue Fang. Denaturing Reversed-Phase HPLC Using a Mobile Phase Containing Urea for Oligodeoxynucleotide Analysis.\u00a0Nucleosides, Nucleotides and Nucleic Acids<\/em>\u00a02014<\/strong>,\u00a033<\/em>,\u00a0481-488<\/a><\/p>\n

Durga Pokharel, Yinan Yuan, Suntara Fueangfung, Shiyue Fang. Synthetic Oligodeoxynucleotide Purification by Capping Failure Sequences with a Methacrylamide Phosphoramidite Followed by Polymerization.\u00a0RSC Advances\u00a0<\/em>2014<\/strong>,\u00a04<\/em>,\u00a08746\u20138757<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Miguel A. Garcia-Campos, Joe Georgeson, Ronit Nir, Robert Reichelt, Kristin A. Fluke, Donna Matzov, Vinithra Iyer, Brett W. Burkhart, Lauren Lui, Anatoly Kustanovich, Felix Gr\u00fcnberger, Supuni Thalalla-Gamage, Shereen A. Howpay-Manage, Milan Gerovac, Nicolas Alexandre, Yuko Nobe, Jakub S. Nowak, Manoj Perera, Alexander Apostle, Shiyue Fang, Sebastian Glatt, Ghil Jona, S\u00e9bastien Ferreira-Cerca, J\u00f6rg Vogel, Masato Taoka,… Continue reading Publications<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-9","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/pages\/9","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/comments?post=9"}],"version-history":[{"count":172,"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/pages\/9\/revisions"}],"predecessor-version":[{"id":1167,"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/pages\/9\/revisions\/1167"}],"wp:attachment":[{"href":"https:\/\/chem.sites.mtu.edu\/fang\/wp-json\/wp\/v2\/media?parent=9"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}