{"id":6,"date":"2016-08-22T12:17:52","date_gmt":"2016-08-22T16:17:52","guid":{"rendered":"http:\/\/chem.sites.mtu.edu\/dam\/?page_id=6"},"modified":"2023-04-01T10:47:21","modified_gmt":"2023-04-01T14:47:21","slug":"research","status":"publish","type":"page","link":"https:\/\/chem.sites.mtu.edu\/dam\/research\/","title":{"rendered":"Research"},"content":{"rendered":"\n

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Current Projects<\/h1>\n\n\n

Project 1. “Ca<\/span>pture and Release” (CaRe): A new method to detect and purify glycan-binding proteins (GBPs) and glycoconjugates.<\/span><\/h1>\n

Welch CJ, Talaga ML, Kadav PD, Edwards JL, Bandyopadhyay P, Dam TK. A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins. J Biol Chem<\/strong>. 2020 Jan 3;295(1):223-236. doi: 10.1074\/jbc.RA119.010625<\/a><\/strong>. Epub 2019 Dec 2. PMID: 31792056; PMCID: PMC6952606.<\/p>\n

Welch CJ, Kadav PD, Edwards JL, Krycia J, Talaga ML, Bandyopadhyay P, Dam TK. A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins. Curr Protoc Protein Sci.<\/strong> 2020 Sep;101(1):e113. doi: 10.1002\/cpps.113<\/a><\/strong>. PMID: 32882112.<\/p>\n

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Schematic presentation of the Capture and Release (CaRe) method<\/strong><\/figcaption><\/figure>\n\n\n\n

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Cover of Current Protocols in Protein Science<\/strong><\/p>\n\n\n\n

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Project 2. Discovery of bioactive compounds from plants<\/mark><\/h1>\n\n\n\n

HelyX: A New Cytotoxic Bioactive Compound<\/em> <\/h2>\n\n\n\n

Project 3. Interaction of Galectin-3 with Proteoglycans and Glycosaminoglycans<\/mark><\/h1>\n\n\n\n

Talaga ML, Fan N, Fueri AL, Brown RK, Bandyopadhyay P, Dam TK. Multitasking Human Lectin Galectin-3 Interacts with Sulfated Glycosaminoglycans and Chondroitin Sulfate Proteoglycans. Biochemistry<\/strong>. 2016 Aug 16;55(32):4541-51. doi: 10.1021\/acs.biochem.6b00504<\/strong><\/a>. Epub 2016 Aug 2. PMID: 27427828.<\/p>\n\n\n\n

Edwards JL, Kadav PD, Bandyopadhyay P, Dam TK. Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein. Methods Mol Biol<\/strong>. 2022;2442:137-150. doi: 10.1007\/978-1-0716-2055-7<\/strong>_8<\/a>. PMID: 35320524.<\/p>\n\n\n

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ITC binding profiles of full-length human Gal-3 with (a) chondroitin sulfate C, and (b) chondroitin sulfate B. (c) Binding of Gal-3 CRD with chondroitin sulfate C. (d) Binding of full-length human Gal-3 with bovine proteoglycan.<\/strong><\/figcaption><\/figure>\n<\/div>\n\n
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Gal-3 binds to GAG chains of proteoglycans by a process<\/strong>
called \u201cbind and jump\u201d<\/strong><\/figcaption><\/figure>\n<\/div>\n\n\n

Completed Projects<\/h1>\n\n\n\n

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Project 1. Interaction of two major thyroid cancer biomarkers<\/mark><\/h1>\n\n\n\n

Fan N, Talaga ML, Brown RK, Fueri AL, Bandyopadhyay P, Dam TK. Glycan-Dependent Mutual and Reversible Sequestration of Two Thyroid Cancer Biomarkers. Thyroid<\/strong>. 2015 Dec;25(12):1339-46. doi: 10.1089\/thy.2015.0105<\/strong><\/a>. Epub 2015 Nov 5. PMID: 26421933.<\/p>\n\n\n\n

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Glycan mediated homeostasis of thyroid cancer biomarkers thyroglobulin and Gal-3<\/strong><\/p>\n\n\n\n

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Project 2. Scaffolds of glycoconjugates modulate their interactions with lectins<\/mark><\/h1>\n\n\n\n

Talaga ML, Fan N, Fueri AL, Brown RK, Chabre YM, Bandyopadhyay P, Roy R, Dam TK. Significant other half of a glycoconjugate: contributions of scaffolds to lectin-glycoconjugate interactions. Biochemistry<\/strong>. 2014 Jul 15;53(27):4445-54. doi: 10.1021\/bi5001307<\/strong><\/a>. Epub 2014 Jul 3. PMID: 24950232.<\/p>\n\n\n

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Proposed mechanism of scaffold mediated functional diversification of glycoconjugates<\/strong><\/h2>\n","protected":false},"excerpt":{"rendered":"

Current Projects Project 1. “Capture and Release” (CaRe): A new method to detect and purify glycan-binding proteins (GBPs) and glycoconjugates. Welch CJ, Talaga ML, Kadav PD, Edwards JL, Bandyopadhyay P, Dam TK. A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins. J Biol Chem. 2020 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-6","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/pages\/6","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/comments?post=6"}],"version-history":[{"count":24,"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/pages\/6\/revisions"}],"predecessor-version":[{"id":247,"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/pages\/6\/revisions\/247"}],"wp:attachment":[{"href":"https:\/\/chem.sites.mtu.edu\/dam\/wp-json\/wp\/v2\/media?parent=6"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}