{"id":670,"date":"2023-11-07T17:58:45","date_gmt":"2023-11-07T17:58:45","guid":{"rendered":"https:\/\/chem.sites.mtu.edu\/amidities\/?page_id=670"},"modified":"2023-11-07T17:58:45","modified_gmt":"2023-11-07T17:58:45","slug":"polymerizable-dt-pdt-phosphoramidite","status":"publish","type":"page","link":"https:\/\/chem.sites.mtu.edu\/amidities\/polymerizable-dt-pdt-phosphoramidite\/","title":{"rendered":"Polymerizable dT (PdT) phosphoramidite"},"content":{"rendered":"\n<p>Using a polymerizable phosphoramidite as the coupling agent in the last synthetic cycle of automated oligodeoxynucleotide (ODN) synthesis, a polymerizable group (i.e. methacrylamide group) can be attached to the full-length ODN (but not to the failure ODNs). After cleavage and deprotection, the full-length ODN is co-polymerized into an insoluble polyacrylamide gel (the failure ODNs are not). The failure ODNs and other impurities are washed away with water. The full-length ODN is then cleaved and extracted from the gel.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Capable of isolation of full-length ODN from complex crude mixture<\/li>\n\n\n\n<li>Useful for the synthesis and purification of long ODNs<\/li>\n\n\n\n<li>Synthesis and purification of up to 400-mer has been demonstrated<\/li>\n\n\n\n<li>Overcoming challenges in the synthesis of genes containing long repeats and stable higher order structures that cannot be overcome using traditional PCR assembly methods<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"360\" height=\"363\" src=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdT.jpg\" alt=\"\" class=\"wp-image-671\" srcset=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdT.jpg 360w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdT-298x300.jpg 298w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdT-150x150.jpg 150w\" sizes=\"(max-width: 360px) 100vw, 360px\" \/><\/figure>\n\n\n\n<p>Molecular Formula: C<sub>55<\/sub>H<sub>75<\/sub>N<sub>6<\/sub>O<sub>12<\/sub>P<\/p>\n\n\n\n<p>Molecular Weight: 1043.21<\/p>\n\n\n\n<p>CAS Number: 1818326-09-8<\/p>\n\n\n\n<p>Purity: &gt;95% (<sup>1<\/sup>H NMR)<\/p>\n\n\n\n<p>Solubility: soluble in CH<sub>3<\/sub>CN and CH<sub>2<\/sub>Cl<sub>2<\/sub><\/p>\n\n\n\n<p>Recommended Storage Temperature: \u201320 <sup>o<\/sup>C or lower<\/p>\n\n\n\n<p>Shipping Temperature: room temperature<\/p>\n\n\n\n<p>Suggested Amount per Experiment: 25 \u00b5mol (26 mg) for each 1 \u00b5mol oligo synthesis<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Using a polymerizable phosphoramidite as the coupling agent in the last synthetic cycle of automated oligodeoxynucleotide (ODN) synthesis, a polymerizable group (i.e. methacrylamide group) can be attached to the full-length ODN (but not to the failure ODNs). After cleavage and deprotection, the full-length ODN is co-polymerized into an insoluble polyacrylamide gel (the failure ODNs are [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-670","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/670","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/comments?post=670"}],"version-history":[{"count":1,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/670\/revisions"}],"predecessor-version":[{"id":672,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/670\/revisions\/672"}],"wp:attachment":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/media?parent=670"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}