{"id":466,"date":"2021-05-17T12:21:16","date_gmt":"2021-05-17T12:21:16","guid":{"rendered":"https:\/\/chem.sites.mtu.edu\/amidities\/index.php\/home-2\/"},"modified":"2023-11-15T19:38:48","modified_gmt":"2023-11-15T19:38:48","slug":"home","status":"publish","type":"page","link":"https:\/\/chem.sites.mtu.edu\/amidities\/","title":{"rendered":"Home"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"466\" class=\"elementor elementor-466\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2f15eec elementor-section-height-min-height elementor-section-items-bottom elementor-section-boxed elementor-section-height-default\" data-id=\"2f15eec\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-609973d\" data-id=\"609973d\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9633795 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"9633795\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bc18369 elementor-widget elementor-widget-heading\" data-id=\"bc18369\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Amidites<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-4d8a723 e-flex e-con-boxed e-con e-parent\" data-id=\"4d8a723\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-35445ee elementor-widget elementor-widget-image\" data-id=\"35445ee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"221\" src=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1024x221.jpg\" class=\"attachment-large size-large wp-image-648\" alt=\"\" srcset=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1024x221.jpg 1024w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-300x65.jpg 300w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-768x166.jpg 768w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1536x332.jpg 1536w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT.jpg 1680w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-eaab567 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"eaab567\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-cff914a\" data-id=\"cff914a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b2e4133 elementor-widget elementor-widget-text-editor\" data-id=\"b2e4133\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Polymerizable deoxynucleoside (PdN including PdA, PdC, PdG and PdT) phosphoramidites are useful for the synthesis of long oligos. Isolation of full-length 400-mer oligos from the complex mixture of chemical synthesis using the catching-by-polymerization (CBP) technology enabled by PdN has been demonstrated recently (<a href=\"https:\/\/chemrxiv.org\/engage\/chemrxiv\/article-details\/6522e6c545aaa5fdbb8e7767\">ChemRxiv<\/a>). Many genes and genomes contain difficult elements such as long repeats and stable higher order structures, and thus are difficult or impossible to construct using known technologies such as PCR assembly. The long oligos made available by the PdN phosphoramidites and CBP oligo purification technology provide an opportunity to address the problem. We proudly offer the PdN phosphoramidites to the communities of synthetic biology, protein engineering, and beyond.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-d55f019 e-flex e-con-boxed e-con e-parent\" data-id=\"d55f019\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a03caa5 elementor-widget elementor-widget-image\" data-id=\"a03caa5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"221\" src=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1-1024x221.jpg\" class=\"attachment-large size-large wp-image-650\" alt=\"\" srcset=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1-1024x221.jpg 1024w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1-300x65.jpg 300w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1-768x166.jpg 768w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1-1536x332.jpg 1536w, https:\/\/chem.sites.mtu.edu\/amidities\/wp-content\/uploads\/2023\/11\/PdA-PdC-PdG-PdT-1.jpg 1680w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0201f33 elementor-widget elementor-widget-text-editor\" data-id=\"0201f33\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>During oligo (i.e. oligodeoxynucleotide, ODN) synthesis, the failure sequences are capped with Ac<sub>2<\/sub>O and full-length sequences are tagged with a PdN phosphoramidite (Figure). After cleavage and deprotection, the crude contains the full-length ODN (the desired product) with a polymerizable group (i.e. methacryl amide group, red portion in PdN in the Figure), failure ODNs and small molecules. For purification, the full-length ODN is co-polymerized into a polyacrylamide gel. Failure ODNs and small molecules are washed away. The product is cleaved and extracted, and then precipitated with <em>n<\/em>BuOH.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3c1fd71 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3c1fd71\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1ea7e0c\" data-id=\"1ea7e0c\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6bf0335 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"6bf0335\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6d132b5 elementor-widget elementor-widget-heading\" data-id=\"6d132b5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Wish to know more about admissions and updates? Subscribe now!<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d1c03ba elementor-widget elementor-widget-wpforms\" data-id=\"d1c03ba\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"wpforms.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"wpforms-container inline-fields\" id=\"wpforms-15\"><form id=\"wpforms-form-15\" class=\"wpforms-validate wpforms-form\" data-formid=\"15\" method=\"post\" enctype=\"multipart\/form-data\" action=\"\/amidities\/wp-json\/wp\/v2\/pages\/466\" data-token=\"75d5c90490e6b0f6fbeb0abfa76ad7f8\" data-token-time=\"1777031412\"><noscript class=\"wpforms-error-noscript\">Please enable JavaScript in your browser to complete this form.<\/noscript><div class=\"wpforms-field-container\"><div id=\"wpforms-15-field_1-container\" class=\"wpforms-field wpforms-field-email\" data-field-id=\"1\"><label class=\"wpforms-field-label wpforms-label-hide\" for=\"wpforms-15-field_1\">Email <span class=\"wpforms-required-label\">*<\/span><\/label><input type=\"email\" id=\"wpforms-15-field_1\" class=\"wpforms-field-large wpforms-field-required\" name=\"wpforms[fields][1]\" placeholder=\"Email address\" spellcheck=\"false\" required><\/div><\/div><!-- .wpforms-field-container --><div class=\"wpforms-submit-container\" ><input type=\"hidden\" name=\"wpforms[id]\" value=\"15\"><input type=\"hidden\" name=\"page_title\" value=\"\"><input type=\"hidden\" name=\"page_url\" value=\"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/466\"><input type=\"hidden\" name=\"url_referer\" value=\"\"><button type=\"submit\" name=\"wpforms[submit]\" id=\"wpforms-submit-15\" class=\"wpforms-submit\" data-alt-text=\"Sending...\" data-submit-text=\"Subscribe\" aria-live=\"assertive\" value=\"wpforms-submit\">Subscribe<\/button><\/div><\/form><\/div>  <!-- .wpforms-container -->\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-8b7142b e-flex e-con-boxed e-con e-parent\" data-id=\"8b7142b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Amidites Polymerizable deoxynucleoside (PdN including PdA, PdC, PdG and PdT) phosphoramidites are useful for the synthesis of long oligos. Isolation of full-length 400-mer oligos from the complex mixture of chemical synthesis using the catching-by-polymerization (CBP) technology enabled by PdN has been demonstrated recently (ChemRxiv). Many genes and genomes contain difficult elements such as long repeats [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"disabled","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-466","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/466","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/comments?post=466"}],"version-history":[{"count":25,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/466\/revisions"}],"predecessor-version":[{"id":717,"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/pages\/466\/revisions\/717"}],"wp:attachment":[{"href":"https:\/\/chem.sites.mtu.edu\/amidities\/wp-json\/wp\/v2\/media?parent=466"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}