External/Internal Funding
| 2022-2025 | Agency: National Institutes of Health (Grant # 1 R15 GM148964-01) Topic: Are all Protein Aggregates Toxic? Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: : The goal of this project is to identify unique physicochemical properties of aggregates, shared by a large number of structurally diverse proteins (e.g. Aβ42 peptide, insulin, and lysozyme), that are toxic. |
| 2019-2022 | Agency: National Institutes of Health (Grant #2 R15 GM114751-02) Topic: Ratiometric Near-Infrared Fluorescent Probes for Sensitive Detection of Lysosomal and Mitochondrial pH Changes in Live Cells Principal Investigator: Haiying Liu, Ph.D. Co-Investigator: Ashutosh Tiwari, Ph.D. Objective: Develop ratiometric near-infrared fluorescent probes for analysis of lysosomal and mitochondrial pH changes in live cells. |
| 2016-Present | Agency: Alumni Donation Topic: The Protein Misfolding Diseases Research Fund Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: Laboratory research funds for Tiwari lab. |
| 2015-Present | Agency: Alumni Donation Topic: Linda J. Horton Laboratory Research Fund Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: Laboratory research funds for Tiwari lab. |
| 2015-2019 | Agency: National Institutes of Health (Grant #2 R15 GM114751-01) Topic: Ratiometric Near-Infrared Fluorescent Probes for Lysosomal pH in Living Cells Principal Investigator: Haiying Liu, Ph.D. Co-Investigator: Ashutosh Tiwari, Ph.D. Objective: To synthesize and test near-infrared fluorescent probes for lysosomal pH in living cells. |
| 2014-2015 | Agency: MTU Research Excellence Fund Topic: Understanding the Role of Protein Aggregation in Cellular Toxicity Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: To analyze a structurally diverse set of aggregation-prone proteins for their shared biochemical and biophysical properties including stability, post-translational modifications, and aggregation propensity in vitro, and to identify their interacting partners in vivo. |
| 2012-2013 | Agency: MTU Research Excellence Fund Topic: BODIPY-based Polymeric Dyes Bearing Carbohydrate Residues for Detection of Bacteria Principal Investigator: Haiying Liu, Ph.D. Co-Investigator: Ashutosh Tiwari, Ph.D. Objective: To develop a broad new class of BODIPY polymer-based ratiometric biosensors for quick, selective and sensitive detection of E. coli bacteria by using fluorescence resonance energy transfer (FRET). |
| 2011-2013 | Agency: ALS Therapy Alliance Topic: Characterizing the Surface Hydrophobicity of ALS Mutants of SOD1 by Novel Fluorescent Probes Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: To characterize abnormally expossed hydrophobic residues of SOD1 using novel fluorescent probes. |
| 2008-2009 | Agency: The Angel Fund Topic: Altered Metal Binding and Hydrophobic Exposure of ALS mutants of Cu/Zn Superoxide dismutase may be key to Cellular Toxicity Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: To measure the metal (copper and zinc) affinity of purified wild type and ALS mutants of Cu/Zn superoxide dismutase (SOD1) and also define potentially toxic conformations of mutant SOD1 by mapping the exposed hydrophobic amino acids. |
| 2007-2008 | Agency: The ALS Therapy Alliance Topic: Mapping the Aberrant Hydrophobicity of Misfolded SOD1 Mutants that Cause ALS Principal Investigator: Lawrence J. Hayward, M.D., Ph.D. UMMS Co-Investigator: Ashutosh Tiwari, Ph.D. Objective: To map specific residues within SOD1 mutants responsible for abnormal hydrophobic exposure and binding in cellular and rodent models of ALS. |
| 2005-2007 | Agency: Amyotrophic Lateral Sclerosis Association (ALSA) and the ALS Therapy Alliance Topic: Aberrant Hydrophobicity of ALS Mutant Cu/Zn Superoxide Dismutase (SOD1) Variants and its Relation to Toxicity Principal Investigator: Ashutosh Tiwari, Ph.D. Objective: To map regions within SOD1 mutants responsible for abnormal hydrophobic binding and to investigate the nature of these interactions in cellular and rodent models of ALS. |