Funding

External/Internal Funding

2019-2022Agency: National Institutes of Health (Grant #2 R15 GM114751-02)
Topic: Ratiometric Near-Infrared Fluorescent Probes for Sensitive Detection of Lysosomal and Mitochondrial pH Changes in Live Cells
Principal Investigator: Haiying Liu, Ph.D.
Co-Investigator: Ashutosh Tiwari, Ph.D.
Objective: Develop ratiometric near-infrared fluorescent probes for analysis of lysosomal and mitochondrial pH changes in live cells.
2016-PresentAgency: Alumni Donation
Topic: The Protein Misfolding Diseases Research Fund
Principal Investigator: Ashutosh Tiwari, Ph.D.
Objective: Laboratory research funds for Tiwari lab.
2015-PresentAgency: Alumni Donation
Topic: Linda J. Horton Laboratory Research Fund
Principal Investigator: Ashutosh Tiwari, Ph.D.
Objective: Laboratory research funds for Tiwari lab.
2015-2019Agency: National Institutes of Health (Grant #2 R15 GM114751-01)
Topic: Ratiometric Near-Infrared Fluorescent Probes for Lysosomal pH in Living Cells
Principal Investigator: Haiying Liu, Ph.D.
Co-Investigator: Ashutosh Tiwari, Ph.D.
Objective: To synthesize and test near-infrared fluorescent probes for lysosomal pH in living cells.
2014-2015Agency: MTU Research Excellence Fund
Topic: Understanding the Role of Protein Aggregation in Cellular Toxicity
Principal Investigator: Ashutosh Tiwari, Ph.D.
Objective: To analyze a structurally diverse set of aggregation-prone proteins for their shared biochemical and biophysical properties including stability, post-translational modifications, and aggregation propensity in vitro, and to identify their interacting partners in vivo.
2012-2013Agency: MTU Research Excellence Fund
Topic: BODIPY-based Polymeric Dyes Bearing Carbohydrate Residues for Detection of Bacteria
Principal Investigator: Haiying Liu, Ph.D.
Co-Investigator: Ashutosh Tiwari, Ph.D.
Objective: To develop a broad new class of BODIPY polymer-based ratiometric biosensors for quick, selective and sensitive detection of E. coli bacteria by using fluorescence resonance energy transfer (FRET).
2011-2013Agency: ALS Therapy Alliance
Topic: Characterizing the Surface Hydrophobicity of ALS Mutants of SOD1 by Novel Fluorescent Probes
Principal Investigator: Ashutosh Tiwari, Ph.D.
Objective: To characterize abnormally expossed hydrophobic residues of SOD1 using novel fluorescent probes.
2008-2009Agency: The Angel Fund
Topic: Altered Metal Binding and Hydrophobic Exposure of ALS mutants of Cu/Zn Superoxide dismutase may be key to Cellular Toxicity
Principal Investigator: Ashutosh Tiwari, Ph.D.
Objective: To measure the metal (copper and zinc) affinity of purified wild type and ALS mutants of Cu/Zn superoxide dismutase (SOD1) and also define potentially toxic conformations of mutant SOD1 by mapping the exposed hydrophobic amino acids.
2007-2008Agency: The ALS Therapy Alliance
Topic: Mapping the Aberrant Hydrophobicity of Misfolded SOD1 Mutants that Cause ALS
Principal Investigator: Lawrence J. Hayward, M.D., Ph.D. UMMS
Co-Investigator: Ashutosh Tiwari, Ph.D.
Objective: To map specific residues within SOD1 mutants responsible for abnormal hydrophobic exposure and binding in cellular and rodent models of ALS.
2005-2007Agency: Amyotrophic Lateral Sclerosis Association (ALSA) and the ALS Therapy Alliance
Topic: Aberrant Hydrophobicity of ALS Mutant Cu/Zn Superoxide Dismutase (SOD1) Variants and its Relation to Toxicity
Principal Investigator: Ashutosh Tiwari, Ph.D.
Objective: To map regions within SOD1 mutants responsible for abnormal hydrophobic binding and to investigate the nature of these interactions in cellular and rodent models of ALS.