Research

Current Projects

Project 1. “Capture and Release” (CaRe): A new method to detect and purify glycan-binding proteins (GBPs) and glycoconjugates.

Welch CJ, Talaga ML, Kadav PD, Edwards JL, Bandyopadhyay P, Dam TK. A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins. J Biol Chem. 2020 Jan 3;295(1):223-236. doi: 10.1074/jbc.RA119.010625. Epub 2019 Dec 2. PMID: 31792056; PMCID: PMC6952606.

Welch CJ, Kadav PD, Edwards JL, Krycia J, Talaga ML, Bandyopadhyay P, Dam TK. A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins. Curr Protoc Protein Sci. 2020 Sep;101(1):e113. doi: 10.1002/cpps.113. PMID: 32882112.

 

Schematic presentation of the Capture and Release (CaRe) method

Cover of Current Protocols in Protein Science

Project 2. Discovery of bioactive compounds from plants

HelyX: A New Cytotoxic Bioactive Compound

Effect of HelyX on human red blood cells (click to watch the video)

Project 3. Interaction of Galectin-3 with Proteoglycans and Glycosaminoglycans

Talaga ML, Fan N, Fueri AL, Brown RK, Bandyopadhyay P, Dam TK. Multitasking Human Lectin Galectin-3 Interacts with Sulfated Glycosaminoglycans and Chondroitin Sulfate Proteoglycans. Biochemistry. 2016 Aug 16;55(32):4541-51. doi: 10.1021/acs.biochem.6b00504. Epub 2016 Aug 2. PMID: 27427828.

Edwards JL, Kadav PD, Bandyopadhyay P, Dam TK. Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein. Methods Mol Biol. 2022;2442:137-150. doi: 10.1007/978-1-0716-2055-7_8. PMID: 35320524.

ITC binding profiles of full-length human Gal-3 with (a) chondroitin sulfate C, and (b) chondroitin sulfate B. (c) Binding of Gal-3 CRD with chondroitin sulfate C. (d) Binding of full-length human Gal-3 with bovine proteoglycan.
Gal-3 binds to GAG chains of proteoglycans by a process
called “bind and jump”

Completed Projects

Project 1. Interaction of two major thyroid cancer biomarkers

Fan N, Talaga ML, Brown RK, Fueri AL, Bandyopadhyay P, Dam TK. Glycan-Dependent Mutual and Reversible Sequestration of Two Thyroid Cancer Biomarkers. Thyroid. 2015 Dec;25(12):1339-46. doi: 10.1089/thy.2015.0105. Epub 2015 Nov 5. PMID: 26421933.

Click to watch the video

Glycan mediated homeostasis of thyroid cancer biomarkers thyroglobulin and Gal-3

Project 2. Scaffolds of glycoconjugates modulate their interactions with lectins

Talaga ML, Fan N, Fueri AL, Brown RK, Chabre YM, Bandyopadhyay P, Roy R, Dam TK. Significant other half of a glycoconjugate: contributions of scaffolds to lectin-glycoconjugate interactions. Biochemistry. 2014 Jul 15;53(27):4445-54. doi: 10.1021/bi5001307. Epub 2014 Jul 3. PMID: 24950232.

Proposed mechanism of scaffold mediated functional diversification of glycoconjugates